Effects of a Gluten-Free Diet on the Gut Microbiota (2024)

Effects of a Gluten-Free Diet on the Gut Microbiota (1)By Dr. Maho Yokoyama, Ph.D.Reviewed by Michael Greenwood, M.Sc.

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  • Why a Gluten Free Diet?
  • How Does a Gluten-free Diet Affect the Microbiota?
  • What are the Difficulties in Studying the Microbiota?

Effects of a Gluten-Free Diet on the Gut Microbiota (3)

Gluten free. Image Credit: Baibaz / Shutterstock

Why a Gluten Free Diet?

Gluten is a protein found in grains such as wheat, barley, and rye, and is comprised of gliadins. For some people, eating foods that contain gluten can cause digestive problems due to gluten-related disorders.

Gluten-related disorders can range from mild to severe in symptoms, and include celiac disease and non-celiac gluten sensitivity.

For patients suffering with gluten-related disorders, it is necessary to stick to a gluten-free diet. More recently, people who do not suffer from gluten-related disorders have started to adopt a gluten-free diet as part of a lifestyle choice.

The rising popularity of gluten-free diets as a lifestyle choice means that they outnumber the number people who must eat a gluten-free diet for their gluten-related disorder.

How Does a Gluten-free Diet Affect the Microbiota?

The gut microbiota consists of the numerous microorganisms that live in our gut. It has been shown that the gut microbiota is key to protecting our gut against pathogens, and that it has the ability to make and regulate molecules going into the blood.

Effects of a Gluten-Free Diet on the Gut Microbiota (4)

Intestinal microbiome, 3D illustration showing anatomy of human digestive system and enteric bacteria Escherichia coli, E. coli, colonizing jejunum, ileum, other parts of intestine. Gut normal flora - Illustration Credit: Kateryna Kon / Shutterstock

Also, the microbiota has the ability to process molecules that the host does not have the capacity to process. For example, the microbiota can metabolize carbohydrates that the host cannot digest, and produce vitamins that the host cannot.

It is heavily influenced by diet, therefore there have been various studies done to investigate how a gluten-free diet affects the gut microbiota.

Bonder and co. investigated the effect of a short-term gluten-free diet on the microbiota. For this study, the authors recruited 21 adult volunteers who did not suffer from symptomatic gluten-related disorders, and followed them through 4 weeks on a gluten-free diet, 5 weeks on a “wash-out” diet and a further 4 weeks on the individuals’ habitual diet.

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Fecal samples were collected from the volunteers before the gluten-free diet, then weekly during the gluten-free diet and the habitual diet. DNA was then extracted from the stool samples, and the 16S rRNA sequence was determined to see the bacterial composition in the microbiome.

The authors found that while there was no difference in the overall microbiome diversity of individual volunteers, certain species changed in abundance on a gluten-free diet. This included reductions in Veillonellaceae family, Ruminococcus bromii and Roseburia faecis, and increases in Victivallaceae, Clostridiaceae and Coriobacteriaceae families. Most of these species are known to play a role in starch metabolism.

In another study, by de Palma and co., 10 volunteers without symptomatic gluten-related disorders were recruited, and over one month their diet was changed to gluten-free. When the fecal microbiota was analyzed by a combination of techniques, it showed that the number of beneficial bacteria (Bifidobacterium, Lactobacillus) decreased while numbers of potentially pathogenic bacteria (Enterobaceriaceae, E. coli) increased.

In the case of patients with celiac disease, it has been shown that their microbiota is altered: there is a decrease in protective species and an increase in species that could become pathogenic.

Initial studies showed that the imbalance in the microbiota of celiac patients was still somewhat unbalanced after 2 years on a gluten-free diet. This was noticed in the reduced number of potentially pathogenic bacteria, such as E. coli and Staphylococcus species, while the number of protective species such as Bifidobacterium and Lactobacillus was still reduced.

This has been supported by other studies, which showed a reduction in other protective species such as Enterococcus, and an increase in other potentially pathogenic species such as Bacteroides, Salmonella, Shigella, Klebsiella and Enterobacteriaceae family. These changes could also influence inflammation in the gut.

Overall, these studies show that dysbiosis of the gut microbiota seen in celiac disease patients is not fully reversed by the adoption of a gluten-free diet.

In conclusion, these studies show that a gluten-free diet can cause changes in the gut microbiota, and that this could potentially be harmful.

What are the Difficulties in Studying the Microbiota?

While there are consistent findings from these studies, there are some limitations. One limitation is that there are many ways of studying the microbiota, ranging from culture-based methods to DNA sequencing-based methods.

Each technique has its own advantages and disadvantages, and it is difficult to know if differences between these techniques can be accounted for.

Another limitation is the classification of the species in the microbiota: it is traditionally thought that bacteria belonging to the same species have highly similar DNA sequences, but this is not always the case.

Also, some studies group similar 16S rRNA sequences into “operational taxonomic units” (OTUs) based on similarity, but these OUTs may be made up of species that are not related at all.

It should also be noted that genes can pass easily between bacteria, therefore this could create species that are difficult to classify due to the presence of genes that originate from other species.

Sources

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Effects of a Gluten-Free Diet on the Gut Microbiota (2024)

FAQs

Effects of a Gluten-Free Diet on the Gut Microbiota? ›

Overall, these studies show that dysbiosis of the gut microbiota seen in celiac disease patients is not fully reversed by the adoption of a gluten-free diet. In conclusion, these studies show that a gluten-free diet can cause changes in the gut microbiota, and that this could potentially be harmful.

Is gluten-free better for your gut health? ›

Ultimately, avoiding processed foods is far more effective than cutting out gluten altogether. As discussed, many gluten-free products are highly-processed and can be harder to digest, less nutritional, and harmful for the gut microbiome.

Can a gluten-free diet cause digestive problems? ›

However, a gluten-free diet also can cause negative side effects like increased constipation from processed snacks that contain little fiber. You also may find you are more susceptible to gluten cross-contamination.

Does gluten damage the gut lining? ›

Gluten causes significant health concerns in individuals with an intolerance or sensitivity. Research shows gluten can increase intestinal permeability, also known as leaky gut, in people with celiac disease and possibly IBS. However, this does not appear to be the case for people without these conditions.

What are the effects of a gluten-free diet? ›

Some people report feeling dizziness, nausea, extreme hunger and even anxiety and depression when they suddenly go from eating a lot of gluten to being gluten-free. These symptoms usually go away after a few weeks on a gluten-free diet, but talk to your health care provider if they persist.

Does gluten destroy the microbiome? ›

Periodic gluten exposure does not significantly affect the gut microbiome in people with celiac disease (CeD) and non-celiac gluten sensitivity (NCGS) who are on a gluten-free diet, a recent study found.

Is gluten inflammatory for gut? ›

Gluten is a structural component of wheat that may induce gut inflammation and is thus not recommended for individuals with celiac disease. Sustained exposure or abnormal continuous activation of the immune cells to such stimuli can lead to chronic inflammation.

What happens to your digestive system when you stop eating gluten? ›

Many people report digestive symptom improvement within a few days of dropping gluten. Fatigue and brain fog often start getting better in the first week or two. This is a gradual process, though.

What damage does gluten do to the small intestine? ›

It affects the small intestine – the part of the digestive system responsible for absorbing nutrients. If you have coeliac disease, eating gluten – a protein found in wheat, rye, barley, and oats – causes damage to the lining of your small intestine. It also causes inflammation in other parts of your body.

What does a gluten belly look like? ›

FAQs about gluten intolerance symptoms

The intestines make up most of the middle and lower abdomen, so “gluten belly” will look like any other type of lower digestive tract bloating. However, if bloating seems to be a regular or constant problem, that's a possible sign that it may be due to gluten intolerance.

Can you reverse gluten damage? ›

There's no cure for gluten intolerance. But most people find relief from symptoms by following a gluten-free diet. You should work with your healthcare provider and a dietitian to plan your diet.

How long does it take for your gut to heal from gluten? ›

After you stop eating foods with gluten, your symptoms will likely get better in a few days to weeks. Your small intestine should heal completely in 3 to 6 months. In some cases, it can take longer for full healing, even though you may feel better. Your villi will be back and working again.

What does gluten do to your bowels? ›

Individuals with celiac disease experience inflammation in the small intestine after eating gluten. This damages your gut lining and leads to poor nutrient absorption, resulting in significant digestive discomfort and frequent diarrhea or constipation ( 3 ).

What are three cons of gluten-free? ›

A gluten-free diet is based on the products which have a high glycemic index and are devoid of dietary fiber. These foods contain many simple carbohydrates and fats. These factors can give rise to nutritional deficiencies, constipation, and the development of the metabolic syndrome [82].

Why am I so gassy after going gluten-free? ›

Another thing to keep in mind is that gluten-free foods are generally higher in starch and lower in fiber. This can cause problems for some with symptoms such as constipation, gas and bloating.

What are the deficiencies of a gluten-free diet? ›

Vitamin D, iron, B vitamins, calcium, zinc, copper, and vitamins A, E, and K. are often low on a gluten-free diet. But, many healthcare providers do not test for vitamin deficiencies. Because of this, people are taking it upon themselves to prevent the possibility of low vitamin levels.

Are there health benefits to avoiding gluten? ›

A gluten-free diet is also popular among people who haven't been diagnosed with a gluten-related medical condition. The claimed benefits of the diet are improved health, weight loss and increased energy, but more research is needed.

Do you feel better not eating gluten? ›

Many people report digestive symptom improvement within a few days of dropping gluten. Fatigue and brain fog often start getting better in the first week or two. This is a gradual process, though.

Is gluten-free better for IBS? ›

Gluten-free diets may improve symptoms such as abdominal pain, stool consistency, and tiredness, and they can be recommended to patients with IBS. (Strength of Recommendation: B, based on inconsistent, small, randomized controlled trials [RCTs].)

Is gluten-free better for belly fat? ›

Bottom Line. Although a gluten-free diet is the primary treatment for celiac disease and may help to alleviate symptoms in various conditions related to gluten sensitivity, there is currently no evidence showing that a gluten-free diet is effective for weight loss or for general health benefits.

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